Molecular barcoding (DNA barcoding) is a molecular taxonomy technique used to identify and genetically characterise a sample from a ubiquitous gene, generally belonging to the ribosomal, mitochondrial or chloroplast genome (COI, 16S, rbcL, etc.).
DNA barcoding applications
DNA barcoding can be used to:
Identify species from an isolated sample
Compare several samples from the same species or closely related species (phylogeny)
This email address is being protected from spambots. You need JavaScript enabled to view it.
Identification by DNA barcoding
GenoScreen offers a complete molecular barcoding service and can handle your samples from DNA extraction onwards.
Identification of isolated micro-organisms
Identification of animal or plant species
For complex inital matrices (e.g. assemblies of bacterial populations), GenoScreen develops metabarcoding solutions using NGSamplicon sequencing to obtain screenings of samples: soil fauna, intestinal flora, coral reefs, etc.
Identification of isolated micro-organisms
GenoScreen offers you a straight-forward service for identifying your micro-organisms by DNA sequencing and comparison with international databases (NCBI or other).
For species identification, the ribosomal DNA regions are adapted to your micro-organism. In addition, other genomic targets (rpoB, gyrB, panC, etc.) can be used to refine this identification and adapt it to your requirements (for example, discrimination within a group of species sharing the same ribosomal DNA sequence).
Performed on :
- Agar (Petri dishes)
- Liquid culture plate
- FTA card or other matrix
From extracted DNA or thermolysate. We will compare your cleaned and assembled sequences with the international NCBI database.
Once the assignment has been completed, you will receive :
- Raw sequences and chromatograms (.seq/.ab1)
- Sequence consesus (.txt/.FA)
- Analysis report with homology percentages and assignment per sample
Additional solutions
In addition to identification, we are also able to produce phylogenetic trees according to your needs (comparison of samples with each other or within a panel of reference sequences).
Depending on your needs, we can also offer you other targets that are more specific to a particular genus, particularly for species that are too close genetically to the default target (for example, the panC target for the Bacillus genus).
GenoScreen also offers :
MLST typing
for strains whose species is known and for which a typing scheme is available
Whole genome sequencing (WGS)
16S, 18S, 28S, ITS, ...
for more precise characterisation of your micro-organisms
GenoScreen offers you a straight-forward species identification service using DNA sequencing and comparison with international databases (NCBI and/or BOLD).
For species identification, we use target genes from the mitochondrial or chloroplast genome (COI, 16S, CYTB, rbcL, matK, etc.). We have universal primer pairs but we can also adapt to your project, using primers that are more specific to a genus or family depending on the literature.
Other target genes can be used to refine an identification or carry out a more complete phylogenetic study.
In addition to the identification, we are also able to produce phylogenetic trees according to your needs (comparison of samples with each other or with a panel of reference sequences).
PCR amplification on the various targets (6S, 18S, 28S, ITS, etc.) can be carried out using extracted DNA.
We will compare your cleaned and assembled sequences with the international NCBI (or Bold) database.
Once the assignment has been completed, you will receive :
- Raw sequences and chromatograms (.seq/.ab1)
- Consensus sequences (.txt/.FA)
- Analysis report with homology percentages and assignment per sample.
Complementary solutions
In addition to the identification, we can also generate phylogenetic trees according to your needs (comparison of samples with each other or with a panel of reference sequences).
GenoScreen also offers whole genome sequencing (WGS) for a more precise characterisation of your species.