Molecular barcoding (DNA barcoding) is a molecular taxonomy technique used to identify and genetically characterise a sample from a ubiquitous gene, generally belonging to the ribosomal, mitochondrial or chloroplast genome (COI, 16S, rbcL, etc.).
DNA barcoding applications
DNA barcoding can be used to:
- Identify species from an isolated sample
- Compare several samples from the same species or closely related species (phylogeny)
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Identification by DNA barcoding
GenoScreen offers a complete molecular barcoding service and can handle your samples from DNA extraction onwards.
- Identification of isolated micro-organisms
- Identification of animal or plant species
For complex inital matrices (e.g. assemblies of bacterial populations), GenoScreen develops metabarcoding solutions using NGS amplicon sequencing to obtain screenings of samples: soil fauna, intestinal flora, coral reefs, etc.
GenoScreen offers you a straight-forward service for identifying your micro-organisms by DNA sequencing and comparison with international databases (NCBI or other).
For species identification, the ribosomal DNA regions are adapted to your micro-organism. In addition, other genomic targets (rpoB, gyrB, panC, etc.) can be used to refine this identification and adapt it to your requirements (for example, discrimination within a group of species sharing the same ribosomal DNA sequence).
Identification services
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Workflow applied to isolated microorganisms
from isolated colonies
16S, 18S, 28S, ITS, ...
consensus, then comparison
Your samples can be extracted on :
- Agar (Petri dishes),
- Liquid culture plates,
- FTA card or other matrix.
PCR amplification on the various targets (16S, 18S, 28S, ITS, etc.) can be carried out using extracted DNA or thermolysate.
We will compare your purified and assembled sequences with the international NCBI database.
Once the assignment has been completed, you will receive:
- Raw sequences and chromatograms (.seq/.ab1)
- Consensus sequences (.txt/.FA)
- Analysis report with homology percentages and assignments per sample.
Complementary solutions
In addition to the identification, we can also generate phylogenetic trees according to your needs (comparison of samples with each other or with a panel of reference sequences).
Depending on your needs, we can also suggest other targets that are more specific to a particular genus, particularly for species that are too close genetically to the default target (e.g. the genus Bacillus with the panC target).
GenoScreen also offers:
- MLST typing, for strains whose species is known and for which a typing scheme is available
- Whole Genome Sequencing (WGS) for more precise characterisation of your microorganisms.
GenoScreen offers you a straight-forward species identification service using DNA sequencing and comparison with international databases (NCBI and/or BOLD).
For species identification, we use target genes from the mitochondrial or chloroplast genome (COI, 16S, CYTB, rbcL, matK, etc.). We have universal primer pairs but we can also adapt to your project, using primers that are more specific to a genus or family depending on the literature.
Other target genes can be used to refine an identification or carry out a more complete phylogenetic study.
In addition to the identification, we are also able to produce phylogenetic trees according to your needs (comparison of samples with each other or with a panel of reference sequences).
Matrices for animal and plant studies
Whole insects or legs/heads
Hairs/feathers
Extracted DNA
Dried or fresh leaves
Blood
Swabs/FTA cards
Tissues (muscle,
organ, fin)
We can work with a wide range of matrices/samples, both invasive and non-invasive:
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Workflow applied to animals and plants
COI, 16S, CYTB, rbcL, matK, ...
reverse sequencing
consensus, then comparison
We will compare your cleaned and assembled sequences with the international NCBI (or Bold) database.
Once the assignment has been completed, you will receive :
- Raw sequences and chromatograms (.seq/.ab1)
- Consensus sequences (.txt/.FA)
- Analysis report with homology percentages and assignment per sample.
Complementary solutions
In addition to the identification, we can also generate phylogenetic trees according to your needs (comparison of samples with each other or with a panel of reference sequences).
GenoScreen also offers whole genome sequencing (WGS) for a more precise characterisation of your species.