Molecular barcoding (DNA barcoding) is a molecular taxonomy technique used to identify and genetically characterise a sample from a ubiquitous gene, generally belonging to the ribosomal, mitochondrial or chloroplast genome (COI, 16S, rbcL, etc.).

 

DNA barcoding applications

DNA barcoding can be used to:

    • Identify species from an isolated sample
    • Compare several samples from the same species or closely related species (phylogeny)

 

This email address is being protected from spambots. You need JavaScript enabled to view it.

 

Identification by DNA barcoding

GenoScreen offers a complete molecular barcoding service and can handle your samples from DNA extraction onwards.

    • Identification of isolated micro-organisms
    • Identification of animal or plant species

 

For complex inital matrices (e.g. assemblies of bacterial populations), GenoScreen develops metabarcoding solutions using NGS amplicon sequencing to obtain screenings of samples: soil fauna, intestinal flora, coral reefs, etc.

Identification of isolated micro-organisms

GenoScreen offers you a straight-forward service for identifying your micro-organisms by DNA sequencing and comparison with international databases (NCBI or other).

For species identification, the ribosomal DNA regions are adapted to your micro-organism. In addition, other genomic targets (rpoB, gyrB, panC, etc.) can be used to refine this identification and adapt it to your requirements (for example, discrimination within a group of species sharing the same ribosomal DNA sequence).

Identification services

Image is not available
Image is not available
Identification of

bacteria

fungi

yeast

microalgae

Workflow for isolated microorganisms

Extraction of your samples

from isolated colonies

PCR amplification on the appropriate genomic target

16S, 18S, 28S, ITS, ...

Forward/reverse
sequencing

Cleaning and assembly

in consensus, then comparison

Assignment

Our offer

Performed on :
- Agar (Petri dishes)
- Liquid culture plate
- FTA card or other matrix

From extracted DNA or thermolysate. We will compare your cleaned and assembled sequences with the international NCBI database.

Once the assignment has been completed, you will receive :
- Raw sequences and chromatograms (.seq/.ab1)
- Sequence consesus (.txt/.FA)
- Analysis report with homology percentages and assignment per sample

Additional solutions

In addition to identification, we are also able to produce phylogenetic trees according to your needs (comparison of samples with each other or within a panel of reference sequences).

Depending on your needs, we can also offer you other targets that are more specific to a particular genus, particularly for species that are too close genetically to the default target (for example, the panC target for the Bacillus genus).

GenoScreen also offers :

MLST typing

for strains whose species is known and for which a typing scheme is available

Whole genome sequencing (WGS)

16S, 18S, 28S, ITS, ...
for more precise characterisation of your micro-organisms

Interested in our solutions for your project?
Contact us and describe your project!
Identification of animal and plant species

GenoScreen offers you a straight-forward species identification service using DNA sequencing and comparison with international databases (NCBI and/or BOLD).

For species identification, we use target genes from the mitochondrial or chloroplast genome (COI, 16S, CYTB, rbcL, matK, etc.). We have universal primer pairs but we can also adapt to your project, using primers that are more specific to a genus or family depending on the literature.

Other target genes can be used to refine an identification or carry out a more complete phylogenetic study.

In addition to the identification, we are also able to produce phylogenetic trees according to your needs (comparison of samples with each other or with a panel of reference sequences).

Matrices for animal and plant studies

Whole insects or legs/heads

Extracted DNA

Hairs/feathers

Blood

Dried or fresh leaves

Tissues (muscle,organ, fin)

Swabs / FTA cards

Workflow applied to animals and plants

Our offer

PCR amplification on the various targets (6S, 18S, 28S, ITS, etc.) can be carried out using extracted DNA.

We will compare your cleaned and assembled sequences with the international NCBI (or Bold) database.

Once the assignment has been completed, you will receive :
- Raw sequences and chromatograms (.seq/.ab1)
- Consensus sequences (.txt/.FA)
- Analysis report with homology percentages and assignment per sample.

Complementary solutions

In addition to the identification, we can also generate phylogenetic trees according to your needs (comparison of samples with each other or with a panel of reference sequences).

GenoScreen also offers whole genome sequencing (WGS) for a more precise characterisation of your species.

Interested in our solutions for your project?
Contact us and describe your project!

By continuing your visit to this site, you accept the use of statistics cookies