Long-read sequencing, also known as Single Molecule Sequencing (SMS), is at the core of 3rd generation sequencing.Powered by Oxford Nanopore Technologies (ONT) and Pacific Biosciences (PacBio), this 3rd generation technology enables the sequencing of large DNA or RNA fragments (theoretically infinite), whereas short-read technologies were limited to fragments of up to 600bp.

 

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When should long read sequencing be used?

In 2022, GenoScreen was certified as a service provider by Oxford Nanopore Technologies. This recognition complements our existing expertise with PacBio technology. By combining these two long-read sequencing approaches, we are able to address your genomics and metagenomics needs with even greater precision.

 

Long-read sequencing applications

Microbial biodiversity

Explore microbial biodiversity with long-read sequencing

At GenoScreen, our expertise is based on the in-depth study of microbial communities using metagenomic approaches. We offer you an innovative approach using full-length sequencing of the 16S gene with our Hi-Res metagenomic approach. This methodology enables more precise taxonomic identification, with the ability to distinguish bacteria not only down to species level, but sometimes even down to strain level for unprecedented resolution. Discover how our advanced technology can open up new perspectives in microbial exploration.

Genomes characterization

Long-read sequencing to better characterize genomes

Whole genome sequencing (WGS), a fundamental approach in genomics, benefits greatly from the capabilities of long-read sequencing. This technology unables the sequencing of long DNA fragments, whether used in isolation in long-read mode, or in combination with short-reads. When used exclusively in long-read mode, it becomes a powerful tool for the detection of structural variations and the generation of continuous assemblies. From circularity for bacteria to chromosomal scale for small eukaryotes, long-read sequencing brings increased precision and resolution. When combined with short-read sequencing, long-read sequencing achieves the highest levels of accuracy. This combination of short- and long-reads produces complete and highly accurate de novo assemblies, with a wide range of applications, including the generation of reference genomes.

Enhance quality control

Enhance quality control with plasmid sequencing

n the field of molecular biology, plasmids play a key part. They are used for a wide range of applications, from protein production to genome engineering and gene therapy. Ensuring the integrity of plasmids is crucial, and this is where long-read sequencing comes in. Unlike traditional Sanger sequencing methods, which only test a restricted part of the plasmid, long-read sequencing enables a complete and simultaneous analysis of the backbone and inserts. It is therefore possible to validate plasmid structure, correct insertion and insert sequence. At GenoScreen, we offer you the opportunity to strengthen your quality control with Oxford Nanopore Technologies' cutting-edge technology.

 

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