Catalogue

GenoBiome® - All-in-one diagnostic solution for microbiome

Genomics has revolutionized the study of microbiomes by enabling in-depth analysis of the DNA of micro-organisms present. Based on this approach, GenoBiome^® makes it possible to characterise microbial diversity, identify specific species and study the interactions between these micro-organisms and their environment.

This knowledge offers new opportunities in developping products respecting symbiotic bacterial communities in different fields of application, such as:

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The integrative solution for studying microbial communities

GenoBiome^® offers a complete range of support services for microbial community analysis, with analytical and bioinformatics protocols designed specifically for the matrix studied (skin, soil, gut).

The aim of this solution is to provide you with the scientific support, technical analysis and flexible assistance needed to lead your project to a comprehensive resolution of your biological inquiries.

Our consultancy services, provided by Ph.Ds in microbial ecology or genomics, foster a holistic approach to scientific research and a focus on the repeatability and reproducibility of experimental outcomes.

Experience interactive reports tailored to your needs, facilitating in-depth analysis and comprehension of complex data. Additionally, benefit from comprehensive support for data reporting, enhancing transparency and clarity in scientific communication.

Interactive reports

Our bioinformatics reports are designed to provide a comprehensive experience, helping you grasp your findings with ease.

• Easy-to-navigate report: Zoom, filter, and explore your data directly within the report, no additional tools needed
• Enriched visualizations: Interactive graphs and tables with clear explanations
• Comprehensive context: Gain a richer understanding with detailed annotations
• Transparent methodology: Access a step-by-step explanation of the analysis methods used

Let our experts guide you through your project

Experimental design

Design your project along qualified microbiome experts, starting from your biological question.

 

 

Sampling strategy

Our support extends to implementing a standardized sampling strategy for reliable data generation and accurate interpretation of results. We also offer support for sample collection with :

    Sampling kit                                             

A sampling kit can be supplied with a conservation protocol to ensure that integrity is maintained during transport.

 

 

 

 

 

 

 

Customized samples analysis

Our wide technical portfolio allow you to answer your projects needs, whether it is based on short read sequencing, long read technology or qPCR analysis.

Our sequencing strategy will be driven by your project features, each aspects from the extraction to the analytical methods will be specifically build around them.

    Extraction methodology

Use of low-biomass extraction methods to isolate and purify DNA or RNA from biological samples with a limited amount of genetic material.

 

 

 

Proprietary analysis pipelines

We provide you with curated bioinformatic tools and databases for microbiome analysis with pipelines developed in-house and adapted to the nature of the studied sample.

   

 

 

 

                                             

Results interpretation

You will be provided comprehensible, ready-to-publish and clear results along with a guided tour to ensure that you get the most out of our analysis.

    Interactive report                        

Regardless of the purpose of your project (research, marketing or regulatory), the results will be suitable for everyone.

 

 

Expert support

Comprehensive scientific, technical, and commercial support throughout your entire project, with PhD-level microbiologist consultations.

    Dedicated partner

You will work with one of our experts who will tailor solutions to your specific needs.

 

 

 

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Skin microbiome analysis

Analysis and characterization of cutaneous microbiomes

The skin's microbiota is made up of billions of microorganisms, covering various species, living in community and closely linked to skin health. Increasingly, the microflora is being studied using genomics solutions, and GenoScreen has developed a complete solution for analysing the skin microbiome. These technologies open up new prospects for the development of cosmetics, skincare products and personalised treatments for atopic skins.

What genomics can bring to skin microbiota understanding ?

Metagenomics is the most complete and cutting-edge approach for characterizating and studying microbiomes, allowing to have a global overview of microorganisms in presence in any sample, and their functional potential.

GenoScreen intervenes at different steps of any research and development project on skin microbiome. Our genomic solutions cover the following purposes :

• Studies of the impact on skin microbiota of skincare product or active ingredient
• Dermatology and Clinical studies in dermocosmetics to assess treatments effects on skin diseases
• R&D exploratory projects focusing on skin microbiota
• Proofs of concept of active ingredients effects on cutaneaous microorganisms
• Scientific communication support with ready-to-publish results
• Claim validation, scientific based, to ensure product interaction with skin microbiome
• Substantiation of « microbiome friendly» claims, proving that the concerned cosmetic product doesn't harm the consumer microbiota and his epidermis health
• Prebiotics, Probiotics, Postbiotics ingredients testing
• Microbial communities exploratory studies throughout R&D collaboration for biomarker detection and focused analysis for instance.

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Our solutions for skin microbiome testing and analysis :

From your biological question to its technological answer, we bring together our technologies and know-how to offer the best solution fitting your project.

Mainly performed on in-vivo human samples, our analysis can be conducted with other models (in-vitro synthetic reconstructed human epidermis, ex-vivo explant tissue cultures).

Metagenomics

The key to understand interactions between the epidermis and its microbial flora.

• Short read targeted metagenomics : The first step into cutaneous microbiota analysis. Supported by an Illumina sequencing platform, short read metagenomics provides an overview of the taxonomic genera present in skin swabbing samples. The identification of cutaneous microflora relies on 16S rDNA sequencing for bacterial targets or 18S/ITS rDNA regions for fungal targets.

• Long read targeted metagenomics : The most appropriate solution for skin microbiome analysis and characterization. Long read metagenomics allows to go further in taxonomic identification, to the species level. A significant advantage when it comes to distinguishing species that are important for skin health. Based on either PacBio or Oxford Nanopore Technologies platforms, it enables a more detailed comparison between the samples.

• Shotgun Metagenomics : Sharp and complete description of all microorganism's genomes in each sample. Shotgun metagenomics provides skin microbiome functional potential, beyond the characterization of all species in a single analysis such as prokaryotes (bacteria, yeasts and other Fungi) and small eurkaryotes. Thanks to Next Generation Sequencing (NGS) on an Illumina platform, this non-targeting approach offers a global overview of the genomes of all organisms constituting the cutaneous microbiota and gives access to relative value quantification of their content and proportions in each sample. With this technology, gene annotation is permitted.

• • Taking into account all microorganisms (all domains, kingdoms and genera combined) in a single analysis
  • Relative quantification of samples’ population
  • Global and complete overview of microbiota’s genome
  • Gene annotation
  • Skin microbiome functional potential

Bioinformatics

• Technical expertise

Our bioinformatics platform works on databases and pipelines specific to the skin microbiomes, developed in-house, allowing powerful analysis of microbial communities.

• Decoding skin microbiome

We supply through interactive reports, a complete picture of cutaneous microbiota, integrating, among other parameters, the following : alpha-diversity, beta-diversity, samples distance and composition comparison.

• Easy to use interface

The interface is both interactive for the user, and on the other hand built on a scientific format, making it easy to understand the content, and suitable for its use into scientific publications and communication clinical studies or regulatory files.

qPCR approach

Design of specific primers for any species or strains, and probes for their quantification in all kind of samples : in vivo , ex vivo , in vitro.

Quantitative real-time PCR allows fast and robust assessment of the effects of different skin care products applications on cutaneous microbiota.

GenoBiome^® Skin

All-in-one solution for in vivo skin microbiome analysis, adaptable to each project, with support and expertise from our team of microbiologists, genomic experts and bioinformaticians. GenoBiome^® Skin is based on a complete pipeline, from the experimental protocol design with our experts, through sequencing and bioinformatics data processing, to the delivery and interpretation of results. A broad range of technological answers applies in the frame of GenoBiome^® such as our metagenomic portfolio and strain-specific customized analysis, constituting the most advanced solution to support your research and development projects on skin microbiota.

• Experimental design for in vivo studies
• Cutaneous sampling standardized protocol
• Supply of collection material (swabs)
• Storage and shipment protocol
• Customized analysis pipeline with low biomass extraction methods and optimized sequencing strategy
• Curated bioinformatic tools and databases for skin microbiome analysis
• Interactive reports, ready-to-publish
• Results interpretation : more than data, answers to your biological questions.
• Support and guidance through the entire process, with regular milestones follow-ups

Interested in our solutions for your project? Contact us and indicate us your future study’s parameters, such as : number of samples, initial protocol (sampling frequency), selected panel, aim of the study (biological question), answer sought and associated technology (which level of analysis are you interested in). Our teams will provide you with a fast and appropriate response.

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Characterization and tracking of isolated microorganisms

By applying a broad panel of genomic technologies and services, GenoScreen provides various solutions in identifying microorganisms via DNA sequencing and comparison with international databases.
These powerful and precised solutions are able to identify and characterize the microorganisms of interest: the species, the sub-species or the strain, typing, tracking and antibiotic resistance.

Identification and characterization

In order to identify a specie, ribosomal DNA regions targeted are adapted to each microorganism.

Additionally, to refine and adapt the identification to your needs, other genomic targets (rpoB and gyrB genes, etc.) are then used (discrimination within a group of species sharing the same ribosomal DNA sequence, for example).

GenoScreen can also leverage its know-how in whole-genome sequencing for a more precised characterization of a strain : virulence, antibiotic resistance, genomic comparisons with the strain library, etc.

Microbial typing and tracking

Our solution precisely characterizes any microorganism at the sub-species or strain level by using standardized, validated molecular methods, such as multilocus sequence typing (MLST) and multiple loci VNTR analysis (MLVA).

The typing schemes in open-access databases (PubMLST, MLST.org and MicrobesGenotyping) are used as references for these analyses.
The analysis can also be performed on a custom typing scheme.

Antibiotic resistance

GenoScreen determines antibiotic resistance profiles via targeted sequencing of genes known to be associated with antibiotic resistance or by whole-genome sequencing. The sequences are compared with open-access databases or the literature data.

In-depth NGS sequencing can detect the presence of low-frequency variants conferring resistance (heteroresistance).

Home GenoScreen Expertise

Expertise

An expertise in the genomic characterization of microorganisms

As an international specialist in microbial genomics, GenoScreen develops fast and precised solutions for characterizing, tracking and typing isolated microorganisms (bacteria, fungi, yeasts, etc.) and analyzing complex microbial florae:

• Scientific and technological services with a high level of expertise.
• Research under contract.

These advanced analytical strategies address many research issues in healthcare and environmental sectors. These techniques can identify, track and characterize microorganisms on the individual or community scale in order to understand their positive or negative impacts on health, safety and well-being of humans, animals and environment.

The analysis of microbial communities (microbiotas)

A microbiota analysis provides information on the entire set of microorganisms (bacteria, yeasts, fungi, algae, etc.) present in a given sample.

This type of analysis is now crucial in the evolution of research in human and animal health, nutrition, environment and biotech.
As a leading provider of genome analysis services, GenoScreen provides powerful, comprehensive solutions for microbiota analysis that will boost your research and give you huge beneficial options in genomics of the future!

Understanding whole ecosystems on the molecular scale

When compared with conventional microbiological tests, molecular analyses of microbial communities are, above all, useful in the study of microorganisms within their ecological niches, i.e. in the absence of culture steps in artificial media that separate the microorganisms from their community and their natural environment.

These microbial ecology analyses provide information on biomass, composition, diversity, functions, interactions between microorganisms and interactions with their habitat.

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