Sanger sequencing is a well-proven, low-throughput analytical solution that is well suited to fragments of all sizes (100-1200 bp read length).
GenoScreen’s Sanger sequencing services can be adapted to suit all types of project, regardless the size, delay time or frequency.
GenoScreen - Services provided
Our responsive teams perform the sequencing from the preparation of your samples until the analysis of your data.
Premium Service: a range tailored to your needs
Take full advantage of our expertise!
The Premium range corresponds to the full, personalized analysis of your samples:
- Purification of PCR products as a free option
- Fluorimetric assay
- Protocol adapted to AT or GC-rich sequences if necessary
- A read length of up to 1200 bp
- Shipping within 24 hours
- Technical support by email/phone
The Premium range gives you access to our hotline: expert scientific support for the analysis of sequence profiles.
| One-Shot | One-Shot Plus | Optimized | |
| Results within 24 to 48 hours after receipt | x | x | x |
| Technical support by email and/or phone | x | x | x |
Accepted sample formats:
|
x | x | x |
| Free sample purification (on request) | x | x | x |
| Reading up to 1200 bp | x | x | x |
| Sequence review and corrections with IUPAC annotations | Semi-automated | Manual | |
| Deliverables | .seq / .ab1 | .seq / .ab1 | .seq / .ab1 QC (Quality control) report with comments for each sequence |
| Reprocessing with optimized protocol (on request) | x | ||
| Monthly activity report (including remaining credits) | x | x | x |
The One-Shot Plus offer gives you the benefit of automated enhancement of your sequences using dedicated software. The One-Shot Plus offer includes :
- Signal optimization,
- Background noise reduction,
- Assignment of indeterminate bases.
The Optimized package gives you access to the QC report and manual proofreading/correction with playback commentary for each sequence.
Economic rangge: handle a portion of the task to benefit from lower fees
GenoScreen's quality and responsiveness to assist you.
| Mix & Run | Run Only |
|---|---|
| You provide the DNA/primer reaction mixture in microplates | |
| Sequencing (BigDye® Terminator) | You perform the sequencing reactions and supply the microplates |
| Clean-up of the sequencing products for gel migration | Clean-up of the sequencing products for gel migration |
| Migration on an ABI 3730 xl system | Migration on ABI 3730 xl system |
Additional analyses
Further on, GenoScreen offers additional services to complement the sequencing steps:
- Primer walking for longer fragments,
- Purification of PCR products by gel cutting,
- Primer design and synthesis,
- Verification of PCR products on QIAxcel prior to sequencing,
- Analysis of results according to IUPAC codes.
GenoScreen's assets
- Flexible sequencing services that meet your needs
- Personalized project monitoring
- An easy-to-use ordering system
- Monthly invoicing or a prepaid customer account
- Free sample shipping by a GenoScreen-approved courier
- Optimized sample protection in a Genobox.
GenoScreen advantages
Do you have any questions about administrative procedures or sample shipping and packaging? Find out more about our Sanger sequencing services in our User Guide or by contacting us below:
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Why choose GenoScreen for your sequencing needs?
- Support right from the formulation of your requirements, to find the technique that best suits your needs
- Access to our hotline, expert scientific support for profile analysis
- A flexible sequencing offer that adapts to your needs
- Personalized follow-up of your projects
- Simple ordering system
- Monthly billing or prepaid account
