Sanger sequencing is a well-proven, low-throughput analytical solution that is well suited to fragments of all sizes (100-1200 bp read length). GenoScreen’s Sanger sequencing services can be adapted to suit all types of project, regardless the size, delay time or frequency.
This email address is being protected from spambots. You need JavaScript enabled to view it.
Sanger sequencing applications
Despite the emergence of new generation technologies (NGS), Sanger sequencing remains a reference method in many fields thanks to its reliability and accuracy.
GenoScreen offer
Flexible and available, our teams carry out sequencing from sample preparation to data analysis. See our documentation below to find out more about our services and to prepare your sequencing request form.
User manual
Form (with purification)
Form (without purification)
Premium range
The Premium offers correspond to a complete and personalised analysis of samples:
- Adaptation of the protocol to GC-rich sequences
- Supply of universal primers
- Sequence reading up to 1200 bp
- Results sent within 24 to 48 hours
The Premium range gives you access to our hotline, by phone and email: expert scientific support for sequence profile analysis.
Workflow tailored to your needs
Purification and dosing
Sequencing reactions
Purification and migration on capillary sequencer
Deliverables with different levels of analysis
After-sales service, eventual reprocessing with optimised protocol
One-Shot
Free purification
Deliverables
.seq / .ab1
Monthly activity report (including remaining credits)
One-Shot Plus
Deliverables
+
Semi-automated review of sequences and corrections with IUPAC annotations
Optimized
Deliverables
+
Manual IUPAC annotations
Reprocessing with optimised protocol (on request)
One-Shot
Purification gratuite
Livrables
.seq / .ab1
Rapport mensuel d'activité (incluant les crédits restants)
One-Shot Plus
Livrables
+
Relecture des séquences et corrections avec annotations IUPAC semi-automatisée
Optimized
Livrables
+
Annotations IUPAC manuelle
Rapport QC (Quality control) relecture manuelle et commentée de chaque séquence avec score qualité
Retraitement avec protocole optimisé (sur demande)
All our offers are open to different sample formats:
- tubes
- strips
- plates, complete or not
Our One-Shot Plus and Optimized offers include additional information:
- Signal optimisation
- Reduction of any backgroun noise
- Assignment of indeterminate bases
- A specific protocol for mutation detection
Additional analyses
GenoScreen offers a range of services to complement sequencing:
Economic range
All the quality and responsiveness of GenoScreen at a low price!
Using your own PCR product + primer mix or even the sequencing reaction you have produced, we can take your samples to a more advanced stage for an optimised price.
GenoScreen advantages
- Flexible sequencing offer that adapts to your needs
-
Personalised monitoring of your projects
-
Simple ordering system
-
Monthly invoicing or prepaid account
-
Free transport of your samples by a GenoScreen-approved operator
-
Optimised protection of samples in Genobox
