Genotyping

MLST/MLVA, microbial typing/tracing solutions

GenoScreen proposes you the opportunity to characterize more accurately your microorganisms (subspecies or strain characterization) using standardized and validated molecular biology methods such as MLST or MLVA (including MIRU-VNTR)

MultiLocus Sequencing Typing (MLST)

The MLST (MultiLocus Sequencing Typing) sequencing typing is the reference technique for the distinction of different strains. This method is based on the house-keeping genes sequencing (house-keeping genes are encoding essential proteins of the bacterium). These sequences have the particularity of presenting a stable polymorphism over time and sufficient enough to distinguish strains from each other.

This approach allows the characterization of a microorganism whose genus (or species) is known in order to identity the species(or subspecies) and this thanks to the sequencing of 7 house-keeping genes.

The MLST by GenoScreen

GenoScreen's MLST service offer is a complete package of services realized from extracted DNA or thermolysate. This includes the PCRs realization, the sequencing on a capillary sequencer, the sequences analysis and the strain assignment..

 Ask for a quotation

Multiple Loci VNTR Analysis (MLVA)

The MLVA (Multiple Loci VNTR Analysis) is a genetic analysis method of VNTR (variable number of tandem repeats) pattern polymorphism based on their count. During a first step, each targeted VNTR locus is amplified by PCR with flanking-region specific primers. The fragments obtained are then separated according to their size by electrophoresis on capillary sequencer. The genotyping of each locus and the results compiled from each sample allow to assign to the strain a specific numerical code for a subspecies.

The MLVA approach allows to characterize a microorganism whose species is known, to identify, at least, the subspecies through allele typing and comparison to the MLVA databases. This method is more selective than the MLST method and does not require a DNA sequencing step. It thus makes it possible to differentiate close subspecies (clonal species).

The MLVA by GenoScreen

GenoScreen's MLVA service offer is a complete package of services realized from extracted DNA or thermolysate. This includes the PCRs realization, the electrophoresis on a capillary sequencer, the data analysis and the strain assignment. 

 Ask for a quotation

The analysis can be performed starting from a customized typing scheme based on your needs, in connection with the data available for your species.

SNP genotyping

GenoScreen - Services provided

GenoScreen offers whole-genome analyses that can detect novel genotype-phenotype associations or known genetic variants.

This technique is based on the detection of a change in a single pair of nucleotides at a particular position. Starting from samples of organic matter, SNP analysis can be used to identify genotypes (people, for example) or build family trees for people or species.

Depending on the number of SNPs studied and the number of samples, GenoScreen can develop additional approaches that address your objectives (TaqMan/KASPar, Fluidigm, Sanger sequencing, etc.):

• NGS for whole-genome analyses (studies of populations or associations and genomic selection)
• Fluidigm Biomark, NGS  and microarrays for genotyping a gene (candidate regions)
• Real-time PCR and Sanger sequencing for analyzing specific individual SNPs (in pharmacogenetics and diagnostics).

For new SNP discovery, GenoScreen offers RAD-Seq approaches. This technology reduces the complexity of the analyses and scans a small fraction (0.1-10%) of a selected genome, for genotyping and/or genetic marker discovery.

The analysis of high-throughput sequencing data enables the identification of a large quantity of genetic variants (such as SNPs).

Ask for a quotation

SSR microsatellite genotyping

This kind of genotyping is based on polymorphism of microsatellites found in the genome.

GenoScreen - Services provided

Identifying and genotyping

The identification and genotyping are essential in the discovery of genetics variations, specially in the studies of the populations and pathologies.

These analyses are extremely informative for many key scientific activities:

• Food traceability.
• Biomarker development and validation (screening/diagnostics, functional genomics, trait prediction models, etc.).
• Studies of genetic diversity and evolution (phylogeny, etc.).
• Identification of individuals (criminals, bodies) or links between individuals (paternity tests).
• Genomic selection (agriculture, animal husbandry, etc.).

Ask for a quotation